Date of Award
Spring 5-12-2021
Document Type
Thesis
Degree Name
Bachelor of Arts
Department
Biochemistry & Molecular Biology
First Advisor
Dr. Joan Slonczewski
Abstract
Multidrug efflux pumps allow bacterial cells to regulate their internal environment through the removal of toxic substances. AcrAB-TolC the most well-studied multidrug efflux pump in Escherichia coli, exports a wide range of substances and is upregulated by the plant hormone salicylate. Other efflux pumps, some with substantial homology to AcrAB-TolC, may have unique, unexplored functions that select for their persistence within the E. coli genome. Using flow cytometry, knock-out strains of multidrug efflux pumps were competed against an ancestral strain in a range of environmental conditions including bile acids, salicylate, and low-pH exposure to uncover their selection patterns. As expected, AcrAB-TolC is the major efflux mechanism of bile acids and underwent positive selection in the presence of bile acids and the proton motive force uncoupler salicylate. However, the MdtEF-TolC and EmrAB-TolC efflux pumps were not selected with bile acids. MdtEF-TolC was positively selected only with low-pH exposure combined with bile acids. Although AcrAB-TolC was also found to be selected with low pH-exposure in bile acids and salicylate, it was not found to have acid-dependent relative fitness like that of MdtEF-TolC. This finding indicates that MdtEF-TolC acts as an acid-dependent pump within E. coli’s Gad acid fitness island. The selection pattern for MdtEF-TolC differed from the selection pattern seen with the deletion of a majority of the gene island, suggesting that other genes within this region contribute to its selection in bile acids. Altogether, this research shows that even structurally similar efflux pumps can be selected under different environmental conditions.
Recommended Citation
Schaffner, Samantha, "Fitness Tradeoffs of Multidrug Efflux Pumps in Escherichia coli K-12 Assessed Through Flow Cytometry Competition Assays" (2021). Honors Theses. 270.
https://digital.kenyon.edu/honorstheses/270
Rights Statement
All rights reserved. This copy is provided to the Kenyon Community solely for individual academic use. For any other use, please contact the copyright holder for permission.