Document Type
Poster
Publication Date
Summer 2025
Abstract
Many plants use seasonal cues, such as day length or temperature, to regulate theirreproductive cycles. The genetic components of these regulatory systems are well understood in flowering plants; however, it is unclear whether these systems extend to non-flowering plants. Previous work with the model moss Physcomitrium patensidentified the gene RAMPANT INDUCTION OF GAMETANGIA (RIG) 1as a component of the seasonal reproductive system. When RIG1is knocked out, P. patensreproduces constitutively even in the absence of proper seasonal cues. To better understand this mechanism, we aimed to identify where the Rig1 protein is located. Over the summer, we worked toward inserting a fluorescent tag at the RIG1gene, as well as its two closest homologs, using CRISPR-Cas9 homology directed repair (HDR). Upon successful insertion of the fluorescent tags, future work will proceed with observing localization of these proteins through visualization under a fluorescent microscope.
Recommended Citation
Suhm, Gabriel, "Fluorescent Tagging of Rig Genes to Understand Seasonal Reproductive Regulation in P. patens" (2025). Kenyon Summer Science Scholars Program. Paper 779.
https://digital.kenyon.edu/summerscienceprogram/779
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