Vector construction for the Constitutive and Phloem-specific Overexpression of a truncated P-type H+-ATPase, AHA3, in Arabidopsis thaliana

Authors

Xinyi Liu, Kenyon CollegeFollow
Tiffany Yang, Kenyon CollegeFollow
Kamesh Regmi, Kenyon CollegeFollow

Document Type

Poster

Publication Date

Summer 2025

Abstract

Arabidopsis thaliana genome harbors 11 P-type H+-ATPase encoding genes, of which Autoinhibited H+-ATPase 3 (AHA3) is involved in powering the secondary active transport of sugars into the phloem (Figure 1)1. All Ptype H+-ATPases have a C-terminal autoinhibitory domain that negatively regulates their activity2,3. We hypothesize that the phloem-specific and constitutive overexpression of AHA3 with a C-terminal deletion (tAHA3) in Arabidopsis will enhance the flux of sugars out of photosynthetically active leaves. We constructed two chimeric plasmids that will allow us to overexpress tAHA3 in a constitutive (p35S::tAHA3) or a phloem-specific (pCOYMV::tAHA3)4 manner, along with the corresponding empty vector controls.

Recommended Citation

Liu, Xinyi; Yang, Tiffany; and Regmi, Kamesh, "Vector construction for the Constitutive and Phloem-specific Overexpression of a truncated P-type H+-ATPase, AHA3, in Arabidopsis thaliana" (2025). Kenyon Summer Science Scholars Program. Paper 747.
https://digital.kenyon.edu/summerscienceprogram/747