Reduced Blood-to-Tissue Albumin Movement After Plasmapheresis

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We tested the hypothesis that a decrease in the blood-to-tissue movement of albumin contributes to the recovery of plasma albumin and plasma volume after acute plasma protein depletion (plasmapheresis). Awake and unrestrained male Sprague–Dawley rats (220–320 g) fitted with jugular catheters were plasmapheresed, and plasma volume, plasma albumin, and total plasma protein content were measured at 1, 5, 24, and 48 h postplasmapheresis. Plasma volume recovered to baseline within 1 h (4.6 ± 0.42 vs. 4.7 ± 0.46 mL/100 g body weight (bw), remained at baseline from 5 h to 24 h but increased to 5.5 ± 0.57 mL/100 g bw at 48 h (P < 0.05). Plasma albumin and total protein content recovered rapidly but remained below baseline levels at 1 h (10.05 ± 0.98 vs. 12.33 ± 1.29 and 19.75 ± 1.75 vs. 24.73 ± 2.56 mg/100 g bw, respectively). Plasma protein content returned to baseline by 5 h of recovery. Tissue uptake of I125-labeled albumin decreased in the heart, skin, skeletal muscle, and small intestines of plasmapheresed rats (P < 0.05). These data support the hypothesis that a reduction in albumin efflux from the vascular space contributes to the recovery of plasma albumin and total protein content during plasma volume recovery and eventual expansion after plasmapheresis.


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